Synthesis of an eco-friendly and efficient antibacterial and antifouling coatings is presented by exploiting urushiol, a natural varnishing material. Since urushiol has inherent outstanding surface-protecting and antimicrobial properties, a series of poly (methyl methacrylate)-urushiol polymer compositions were prepared and fabricated into films. The prepared films were subjected to antimicrobial and antifouling studies. The polymer systems were characterized by various physico-spectroscopic techniques such as 1H NMR, Fourier transform infrared spectroscopy, differential scanning calorimetry, atomic force microscopy, and thermal gravimetric analysis. The confocal laser scanning micrographs, obtained for Pseudomonas aeruginosa biofilm formation, demonstrated an excellent antimicrobial response of the urushiol-incorporated polymers against this bacterial strain. We also demonstrated an inhibitory attachment effect against Navicula incerta, a fouling microalgal strain.