Advertisements

RUDN University chemists to obtain new material for antibacterial food coatings based on natural polymer chitosan

RUDN University chemists have developed a simple and convenient method for producing derivatives of the natural polymer chitosan. These derivatives are non-toxic and have a pronounced antibacterial activity at the level of modern antibiotics. These substances can be used in the production of antibacterial protective films for food. The article is published in the journal Food Chemistry.

Preservatives are widely used in the food industry. They are necessary to extend the shelf life of products. On the other hand, preservatives reduce food quality. Some of them can cause allergies (benzoic acid) or be toxic (nitrates, nitrites). Synthetic waxy substances used to coat fruits can be carcinogenic (biphenyl is prohibited in the US and EU). This explains the importance of finding new preservatives that are effective and safe.

Chitosan is a natural polymer derived from chitin, the main component of insect and crustacean shells. It has antibacterial properties and is already used in the food industry for packaging and coating products with a protective film. However, its activity is far inferior to antibiotics.

To obtain non-toxic and highly active derivatives of chitosan, RUDN University chemists Andreii Kritchenkov and Margarita Kurasova and their colleagues have modified chitosan with organic azides by “sewing” them to the polymer chain. Azides are derivatives of hydrazoic acid (HN3). Both organic and inorganic azides are toxic, but their compounds with chitosan are not poisonous. Antibacterial activity of the obtained substances, azido chitosan, was significantly higher than that of chitosan.

The experiment showed that samples of polymers based on chitosan had antibacterial activity at the level of antibiotics. The researchers compared this parameter for azido chitosan with the two most common antibiotics—ampicillin and gentamicin.

Antibacterial activity was determined by diffusion into the agar (nutrient) layer. A disk of filter paper impregnated with the test substance (or comparison antibiotic) was placed on the agar layer sown with a bacterial culture. The substance penetrates the agar, killing bacteria or slowing their reproduction. As a result, a growth suppression zone is formed around the disk, which differs from the rest of the agar surface visually. The value of antibacterial activity is estimated by the diameter of the formed zones. Toxicity was determined by a standard colorimetric test.

An obtained derivative of chitosan—azido chitosan—prevented Staphylococcus aureus growth in a zone of 26 mm in diameter (the indicator for ampicillin was 30 mm), and of 18 mm for Escherichia coli (in gentamycin it was 22 mm).

That is, the polymer has almost the same antibacterial activity as antibiotics. This is extremely important because the use of antibiotics as components of antibacterial films is highly unacceptable because it leads to the emergence of resistant strains of bacteria. Chitosan compounds do not have this disadvantage.

New chitosan derivatives can be used as harmless and effective preservatives to create protective films for food on this basis.

Story Source:

Materials provided by RUDN University

Note: Content may be edited for style and length.

Journal Reference:

  1. ndreii S. Kritchenkov et al, Novel non-toxic high efficient antibacterial azido chitosan derivatives with potential application in food coatings, Food Chemistry (2019). DOI: 10.1016/j.foodchem.2019.125247

PCE NEEDS YOU

PCE needs your support to continue activities and to improve services. Therefore, please finance PCE by paying your participation to PayPal account below. It is not necessary to be registered on PayPal to use this service. Thanks in advance!

$5.00

Note to readers:

Please follow us on LinkedIn and Twitter and subscribe to our website and receive notifications of new posts by email.

Please click the share buttons below and make a comment.

Advertisements
Advertisements

Leave a Reply

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Advertisements
Advertisements
Advertisements
Advertisements
Advertisements
%d bloggers like this: